Selected lactobacilli strains inhibit inflammation in LPS-induced RAW264.7 macrophages by suppressing the TLR4-mediated NF-κB and MAPKs activation

Probiotics are known to provide the host with immune-modulatory effects, which is widely used for treatment of inflammatory bowel disease (IBD), diarrhea, and ulcers.Lactobacillus acidophilus KLDS 1.0901, Lactobacillus helveticus 1.8701, plantarum 1.0318 were isolated from Chinese fermented dairy food antibacterial activity, antioxidant immunomodulatory respectively. This study evaluated anti-inflammatory potential tested strains in LPS (Lipopolysaccharide)-induced RAW264.7 cells. The results showed that all could inhibit mRNA expression iNOS COX-2 reduce concentration NO PGE2 production. Furthermore, markedly reduced proinflammatory cytokines' production (TNF-α, IL-1β, IL-6). Moreover, these may be associated inhibiting TLR4-mediated NF-κB MAPKs signaling pathway activation. These indicated L. possesses an a molecular basis regarding development functional probiotic products.